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Figure 6
Comparing density of jack bean urease; main-chain density of jack bean urease in a β-sheet fold. (a) PASR-processed map, (b) super resolution map (original data), and (c) map at the physical Nyquist limit. Note the two main-chain density breaks (red arrows) present in the downsampled data, which are not evident for PASR or super resolution. (d) Another region of jack bean urease, focusing on an α-helix with side chains also shown. Clear side-chain density is evident for PASR (d) and super resolution data (e), commensurate with the reported resolution. The Nyquist-frequency-limited data (f) show ambiguous density for side chains, particularly the two glutamine residues and the arginine residue. Fitted PDB ID: 31a4. Once again, here, PASR is clearly competitive with super resolution acquisition, and a large improvement over data limited by the physical sampling frequency. Density displayed at 5σ. Scale bars are 1 nm.

IUCrJ
Volume 13| Part 5| September 2026| Pages 589-605
ISSN: 2052-2525
https://doi.org/10.1107/S2052252526005348