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Figure 7
The improvement in resolution with PASR for apoferritin when acquired with conditions that image almost an entire grid hole. (a) FSC curves of the 4K sampled original data (mustard yellow curve), 8K sampled original data (black curve) and PASR-processed data (blue curve). FSC = 0.143 is indicated with a dashed grey line and the physical Nyquist limit is indicated with a dashed green line. This is a `worst case scenario' for PASR, approximately 0.2 Å lower resolution than `native' super resolution data. The same data processed with Cryo­SPARC are approximately 0.15 Å lower resolution than the native super resolution data. (b) Monomer of apoferritin with monomer rigid-body fitted, (c) the same monomer but resampled to the same pixel spacing as PASR/super resolution data, showing no visible improvement in map clarity, (d) monomer of apoferritin with monomer rigid-body fitted from super resolution (8K sampling) processing, and (e) monomer of apoferritin with monomer rigid-body fit from PASR processing. (f–i) Example side-chain densities for each of (b–e). PASR is clearly competitive with the super resolution (8K) sampled data. Density displayed at 5σ.

IUCrJ
Volume 13| Part 5| September 2026| Pages 589-605
ISSN: 2052-2525
https://doi.org/10.1107/S2052252526005348