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Figure 8
The improvement in resolution with PASR for Melbournevirus, which had originally been limited by sampling frequency during acquisition. (a) Gold-standard FSC curves of the fivefold block, showing the potential improvement from application of PASR. Original curve (mustard yellow line), PASR FSC before Bayesian polishing is carried out (pale blue line) and PASR FSC (black line). (b) Locally filtered map coloured by local resolution of a central slice of the original fivefold block. (c) Locally filtered map coloured by local resolution of a central slice of the PASR fivefold block. Maps shown at 3σ. Scale bars are 5 nm. Colour scales are identical and the colours range from 3.2 Å (blue) through to 6.2 Å (red). The improvement in main-chain clarity is dramatic in the capsid, making model building for proteins of undetermined sequence much more feasible.

IUCrJ
Volume 13| Part 5| September 2026| Pages 589-605
ISSN: 2052-2525
https://doi.org/10.1107/S2052252526005348