Crystallization of the GTP-dependent transcriptional regulator CodY from Bacillus subtilis

Blagova, E.V.; Levdikov, V.M.; Tachikawa, K.; Sonenshein, A.L.; Wilkinson, A.J.

doi:10.1107/S0907444902018358

Crystallization of the GTP-dependent transcriptional regulator CodY from Bacillus subtilis

E. V. Blagova, V. M. Levdikov, K. Tachikawa, A. L. Sonenshein and A. J. Wilkinson

CodY is a GTP sensor that represses transcription of early stationary phase and sporulation genes in Bacillus subtilis. As nutrients become limiting, GTP levels fall and CodY-mediated repression is relieved. Crystals of CodY have been grown in the presence and absence of GTP from sodium citrate buffered solutions containing lithium sulfate and diffraction data have been collected extending to 3.5 Å spacing.

Keywords: CodY; transcription factors; GTP sensors.

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Crystallization of the GTP-dependent transcriptional regulator CodY from Bacillus subtilis

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