Crystallization and preliminary X-ray crystallographic studies of mouse autocrine motility factor

Mouse autocrine motility factor (mAMF), a tumour-secreted cytokine that stimulates cell migration in vitro and metastasis in vivo, has been crystallized by the hanging-drop vapour-diffusion method. The crystals belong to the monoclinic space group P2₁, with unit-cell parameters a = 69.97, b = 115.88, c = 73.27 Å, β = 101.76°. There are two subunits (one dimer) per asymmetric unit. Complexes with four-, five- and six-carbon carbohydrate phosphate inhibitors have also been crystallized. The crystals diffract to at least 1.8 Å resolution and are suitable for X-ray structure analyses at high resolution.