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R. G. Sierra,
H. Laksmono,
J. Kern,
R. Tran,
J. Hattne,
R. Alonso-Mori,
B. Lassalle-Kaiser,
C. Glöckner,
J. Hellmich,
D. W. Schafer,
N. Echols,
R. J. Gildea,
R. W. Grosse-Kunstleve,
J. Sellberg,
T. A. McQueen,
A. R. Fry,
M. M. Messerschmidt,
A. Miahnahri,
M. M. Seibert,
C. Y. Hampton,
D. Starodub,
N. D. Loh,
D. Sokaras,
T.-C. Weng,
P. H. Zwart,
P. Glatzel,
D. Milathianaki,
W. E. White,
P. D. Adams,
G. J. Williams,
S. Boutet,
A. Zouni,
J. Messinger,
N. K. Sauter,
U. Bergmann,
J. Yano,
V. K. Yachandra and
M. J. Bogan An electrospun liquid microjet has been developed that delivers protein microcrystal suspensions at flow rates of 0.14-3.1 µl min-1 to perform serial femtosecond crystallography (SFX) studies with X-ray lasers. Thermolysin microcrystals flowed at 0.17 µl min-1 and diffracted to beyond 4 Å resolution, producing 14 000 indexable diffraction patterns, or four per second, from 140 µg of protein. Nanoflow electrospinning extends SFX to biological samples that necessitate minimal sample consumption.
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