The use of MULTAN to locate the positions of anomalous scatterers

Observed anomalous scattering differences have been used with the direct-methods program MULTAN87 to determine the positions of anomalous scatterers in a variety of metalloproteins and a small molecule. The lack of anomalous differences in the centric data did not prevent the determination of the atom positions and the anomalous scatterers were found in all cases. These results show that the method may be useful to determine the positions of anomalous scatterers in the case of multi-site genetically engineered proteins.