A simple technique for consistently obtaining large single crystals of hen egg-white lysozyme in a concentration gradient of NiCl₂

A simple protocol is reported for consistently obtaining large single crystals of hen egg-white lysozyme (HEWL) greater than 2 mm in the longest dimension. Comparative crystallization experiments with different sources of HEWL showed that the simple addition of glass capillaries, `crystal hangers', reduced excessive nucleation and resulted in large single crystals regardless of the commercial source of HEWL.