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Figure 4
(a) An example of X-ray diffraction patterns taken from frog skeletal muscles in the living relaxed state. The pattern was recorded on an image plate with an exposure of 30 min at a camera length of 2 m and a ring current of ∼4 mA. The background intensity was appropriately subtracted for illustration purposes. The fibre axis of the muscle is vertical. M: meridional axis. E: equator. M1–M9: thick-filament-based layer lines indexed on the 42.9 nm repeat. A51, A59: 5.1 and 5.9 nm thin-filament-based layer lines. (b) Intensity profiles on the meridian in the X-ray pattern in (a). Intensities shown have been multiplied by the square of the axial coordinate. M2–M10: thick-filament-based meridional reflections with a basic repeat of 42.9 nm. C: a 44 nm reflection, possibly from C-protein arrangements on the thick filaments. Tn1–Tn3: reflections with a repeat of 38 nm from troponin molecules on the thin filaments. All fine peaks come from the sampling by the long-range periodicities in a sarcomere (see text). Contamination from the second harmonic was ∼5%.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
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