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Figure 1
TK crystals: (a) macroseeded in 1.1 M sodium/potassium tartrate, 2.5%(v/v) ethanol, 25 mM sodium acetate pH 4.9, 25 mM imidazole pH 7.5; (b) 2.5 M sodium acetate, 25 mM sodium acetate pH 4.9, 25 mM imidazole pH 7.5; (c) 1.0 M sodium citrate, 25 mM imidazole pH 7.5, 25 mM sodium acetate pH 4.9; (d) 18%(w/v) PEG-8000, 38 mM imidazole pH 7.5, 12 mM sodium acetate pH 4.9, 0.2 M magnesium chloride. Further details on crystallization conditions are given in Table 1 ![]() |