Figure 1
TK crystals: (a) macroseeded in 1.1 M sodium/potassium tartrate, 2.5%(v/v) ethanol, 25 mM sodium acetate pH 4.9, 25 mM imidazole pH 7.5; (b) 2.5 M sodium acetate, 25 mM sodium acetate pH 4.9, 25 mM imidazole pH 7.5; (c) 1.0 M sodium citrate, 25 mM imidazole pH 7.5, 25 mM sodium acetate pH 4.9; (d) 18%(w/v) PEG-8000, 38 mM imidazole pH 7.5, 12 mM sodium acetate pH 4.9, 0.2 M magnesium chloride. Further details on crystallization conditions are given in Table 1. Multicrystalline clusters such as those in (c) have also been observed from other crystallization conditions. The dimensions of the crystals are indicated by the bar. |