Figure 2
Schematic of the rapid-mixer flow cell for time-resolved measurements by IR spectroscopy. Denatured protein and renaturant separately enter the mixing groove (100 µm wide, 100 µm deep) and the resultant solution flows into the observation channel (200 µm wide, 52 µm deep) bored in one of the two ZnSe windows, through a 254 µm-wide hole in a 120 µm-thick seal. |