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Figure 3
Optical (top) and false-colored IR images of brain tissue from (a) a normal rat and (b) a rat with severe seizure. Tissues were mounted on BaF2 windows. The aperture size was 10 µm × 10 µm (see the green square at the center of the optical image of normal rat tissue). For each data point in the IR map, a spectrum was acquired using 64 co-additions and Happ-Genzel apodization at 4 cm−1 resolution. A background spectrum through a spot without sample was collected for absorbance reference. The map is generated using an amide I to lipid peak height ratio of bands at 1654 and 2922 cm−1, respectively, and the color represents the intensity of the ratio.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 9| Part 4| June 2002| Pages 189-197
doi:10.1107/S0909049502008543
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