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Figure 9
Thin-layer chromatogram of X-irradiated (lanes 3 and 4) and control (non-irradiated, lane 2) samples of DMPC at 34% w/w water to reveal chemical changes induced by continuous exposure to X-rays. The irradiated sample had been in the uncollimated 13.6 keV X-ray beam on the F1 station at CHESS (2 × 1011 photons s−1) for 5.6 min (∼50 Mrad) at 302 K (in the water-stressed Lα phase, lane 3) or 283 K (in the fully hydrated Lβ′ phase, lane 4). Both samples show significant chemical breakdown. The extent of the radiation damage as monitored by X-ray diffraction was recorded after the exposure. The solvent system was chloroform:methanol:water (65:25:4 by vol.). The lipid standards used were 1-palmitoyl-sn-glycero-3-phosphocholine (lyso PC), lane 1, and palmitic acid (FFA), lane 5. Modified from Fig. 6 of Cheng & Caffrey (1996BB2).

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 9| Part 6| November 2002| Pages 333-341
doi:10.1107/S0909049502014528
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