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Figure 2
Structural differences between N2- and He-cooled XI crystals. Using the PDB accession number coordinates from 1XIB (Carrell et al., 1994BB5) for model phases, maps of differences between the experimental data sets were generated using the fo-fo_map utility of CNS (Brunger et al., 1998BB3) from the two data sets statistically detailed in Table 1[link]. (a) 4σ 6 Å resolution difference density in red for a single XI molecule. Note that most of the difference density appears outside the CPK model of the molecule. (b) 4σ 6 Å resolution difference density within a unit cell of an XI crystal. There is a large amount of difference density within the large (40 Å) solvent pore of the unit cell. Images were generated using Turbo-Frodo (Roussel & Cambillau, 1991BB19).

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