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studies of nitrogenase: the MoFe and VFe proteins and the use of crystallographic coordinates in three-dimensional data analysis
aMolecular Biophysics Group, CCLRC Daresbury Laboratory, Warrington, Cheshire WA4 4AD, UK, and bDepartment of Biological Chemistry, John Innes Centre, Norwich NR4 7UH, UK
*Correspondence e-mail: r.w.strange@dl.ac.uk
This paper reports a three-dimensional Klebsiella pneumoniae nitrogenase (Kp1) using the 1.6 Å-resolution crystallographic coordinates. At this resolution, the positions of the heavy (Fe and S) atoms of the cluster are well determined and there is excellent agreement between the crystallographic and models. However, the lighter homocitrate and histidine ligands are poorly determined in the and it is shown that the application of EXAFS-derived distance restraints during the early stages of crystallographic provides a means of substantially improving (by ∼0.1 Å) the final crystallographic model. The consistency of the analysis with the crystallographic information in this case justifies applications of to cases where protein crystal structures are absent. Thus, the VFe protein of V-nitrogenase has been shown by to possess a V-atom site catalytically similar to the well characterized MoFe-nitrogenases, with V replacing Mo.
of the Mo coordination sphere of the FeMoco cluster of the dithionite-reduced MoFe protein fromKeywords: EXAFS; nitrogen fixation; nitrogenase; crystal structure; FeMoco.
1. Introduction
Nitrogenase, the enzyme responsible for biological 2 to ammonia (Burgess & Lowe, 1996; Howard & Rees, 1994). Nitrogenase is found in relatively few groups of bacteria but is responsible for the global cycling of some 108 tonnes of N per year. All N2-fixing organisms have a highly conserved nitrogenase system based on Mo and Fe, but some organisms additionally have alternative nitrogenases based on V and Fe, or on Fe alone (see Eady, 1996). The three nitrogenase systems, although clearly related, are genetically distinct and do not simply arise from the substitution of different metals into the same All three classes of nitrogenase are two-component systems. The Mo-independent nitrogenases were discovered some 16 years ago and have not been as extensively studied as the Mo system, to which they are clearly related. V-nitrogenase and Fe-only-nitrogenase have a VFe protein and an FeFe protein, respectively, and an associated Fe protein. The structural genes for the three systems have been cloned and sequenced and, as shown in Fig. 1, have a high degree of homology. A comparison of the amino-acid sequences of the MoFe, VFe and FeFe proteins revealed that the amino-acid residues of the α and β subunits, which are ligands to the P clusters and FeMoco and which are invariant in all MoFe proteins known, are all also conserved in the other nitrogenases. This result, together with genetic data, which indicate that some of the genes involved in the biosynthesis of FeMoco are required for functional V- and Fe-nitrogenases, provided strong presumptive evidence that they would also contain analogous cofactor centres to FeMoco. These similarities suggest a common mechanism for the activation and reduction of N2. If this is the case, then only the Fe and possibly the S atoms of the cofactor centres can be involved in substrate binding. The role of the hetero-metal (Mo or V) would be in modulating the reactivity of the centre, as is observed in model chemical systems (Evans et al., 1999). However, work on the Fe-only-nitrogenase, particularly X-ray absorption spectroscopy, has been hindered by the lack of a hetero-metal in this system.
catalyses the ATP-dependent reduction of N2. Experimental
K-edge spectra were taken on the 5 T Wiggler station (9.2). A double-crystal Si(220) monochromator was employed with the crystals offset to give 50% of the maximum intensity. This arrangement allows harmonic content level < 0.1%. Data were measured in fluorescence mode using a Canberra 13-element Ge solid-state detector. The detector was calibrated using the Mo Kα fluorescence signals from a 5 mM sample of molybdate. V K-edge data were measured on station 8.1 using a similar fluorescence detector. All measurements were made with samples in a liquid-nitrogen cryostat. Several 1 h scans of each sample were taken and then summed together after examining the output of each detector element. Normalization and background subtraction of the summed data were carried out by standard methods, as previously described. Analysis of the background-subtracted k3-weighted raw was performed with the EXCURV98 program (Binsted & Hasnain, 1996) using fast curved wave theory (Gurman et al., 1984). The phase shifts employed were calculated using Hedin–Lundqvist exchange and correlation potentials.
measurements were carried out at the Synchrotron Radiation Source, Daresbury Laboratory, operating at 2 GeV and an average current of 200 mA. Mo3. Results and discussion
3.1. Mo-nitrogenases
The molybdenum-containing nitrogenase systems are composed of two metalloproteins: the MoFe protein (component 1) and the Fe protein (component 2). One role of the Fe protein is to act as a very specific MgATP-dependent
to the MoFe protein to catalyse the reactionThe MoFe protein contains two unique metallosulfur clusters, viz. the P clusters and the FeMo cofactor centres (FeMoco). The 8Fe7S P clusters are probably the site of initial acceptance of electrons from the Fe protein. The FeMoco centres constitute the substrate binding and reducing sites of the MoFe protein and can be described as [MoFe3S3] and [Fe4S3] clusters linked by three S2− bridges. The Mo atom is also coordinated to homocitrate via hydroxyl and carboxyl O atoms (Fig. 2). Structural characteristics of the FeMoco centre were first provided by (Arber et al., 1988; Cramer et al., 1978) and subsequently confirmed by several crystal structures of MoFe proteins (Bolin et al., 1990; Chan et al., 1993; Georgiadis et al., 1992; Kim & Rees, 1992; Kim et al., 1993; Mayer et al., 1999). The resolution of these crystal structures has been improved with time but is still far from `atomic' (< 1.2 Å). In this paper we report a three-dimensional (Cheung et al., 2000) using the 1.6 Å-resolution crystallographic coordinates (PDB code 1QGU ) for the dithionite-reduced MoFe protein from Klebsiella pneumoniae nitrogenase (Kp1) (Mayer et al., 1999). The results show a good agreement for interatomic distances for most of the cluster atoms except for the low-Z ligands. The application of EXAFS-derived distance restraints during the early stages of crystallographic provides a means of substantially improving (by ∼0.1 Å) the agreement with the final model.
The α2β2 tetramer with the αβ halves related by a non-crystallographic twofold axis, and each αβ dimer contains one FeMoco centre and one P cluster, giving two independent measurements for each interatomic distance. These two sets of measurements were essentially identical (within ±0.04 Å in the worse case) at 1.6 Å resolution (Mayer et al., 1999). For the calculations, the atomic coordinates for a single FeMoco site (Fig. 2) were used without averaging. Table 1 summarizes the details of the interatomic distances for a three-dimensional of the where only the Debye–Waller factors (σ2) were allowed to refine and the distances were fixed at crystallographic values. Also included are the fit parameters obtained from a three-dimensional where interatomic distances were also allowed to change. The two simulations are shown in Fig. 3. In the first the σ2 values of the low-Z atoms have increased to the extent that these atoms no longer make any contribution to the spectrum, which establishes that their distances are incorrect. In the second with more reasonable σ2 terms, these atoms shift significantly; the imidazole N atom moves by ∼0.2 Å while the O atoms of the homocitrate are rotated to give a change in distance of ∼0.1 Å. At the same time, there is little difference between the two refinements for the rest of the cluster: the Mo—S and Mo—Fe distances are within 0.01 Å of their crystallographic values, including the Mo—Fe shell at ∼5 Å. Thus, the agreement between the PX and structures regarding the positions of the heavier, more strongly scattering atoms is better than that for the light atoms.
of Kp1 contains an
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A similar observation has been made for the type-1 copper proteins, like azurins, where the Cu—S(Cysteine) distance is essentially the same by PX and i.e. within ± 0.02 Å) but the low-Z histidine ligands are found to differ by ∼0.1 Å in their distances to the Cu atom, the values from PX being consistently longer (Cheung et al., 2000; Dodd et al., 2000; Murphy et al., 1993). On the other hand, there is no such discrepancy regarding the comparison between small-molecule crystallography and where the distances of light- and heavy-scattering atoms are normally determined to within ±0.02 Å of each other, for example, the Mo—O and Mo—S distances in [Nbun4]2[MoO(mnt)2] (Baugh et al., 1997). The Cambridge Structural Database (Allen & Kennard, 1993) yields six small-molecule structures relevant to the octahedral Mo coordination in FeMoco. These structures have average Mo—S bond lengths in agreement with the and PX results for Kp1 [2.36 (0.02) Å]. On the other hand, the average Mo—O [2.13 (0.04) Å] and Mo—N [2.28 (0.01) Å] bond lengths agree closely with the results for Kp1. This agreement suggests that the distances are more accurately determined by than by crystallography, at least at resolutions of ∼1.6 Å.
(Fitting the measured electron density by refining the structure using the caused some distortion of the imidazole ring of the histidine residue. Consequently, we attempted a series of protocols in order to try to reconcile the PX and data (see Table 2). The coordinates from 1QGU were crudely randomized by applying a shift of +0.1 Å to one coordinate (i.e. x of atom 1) and −0.1 Å to the next (i.e. y of atom 1) and so on, alternating between +0.1 Å and −0.1 Å throughout the whole PDB file. Also, the temperature factors were all reset to 15 Å2 (the value from TRUNCATE was 14.8 Å2). This model was then subjected to in REFMAC (Murshudov et al., 1997) with anisotropic temperature-factor for the Mo atoms. For runs 2 to 4, the starting model was modified by placing the Mo atoms back at their final positions in 1QGU and the Nδ1, O5 and O7 atoms at the distances away from them. Then these models were refined either with tight restraints to the values (between the clusters and their ligands) or with these restraints effectively switched off. The resulting structure places the histidine ligand closer by ∼0.1 Å to the model. The homocitrate O atoms occupy equivalent distances from the Mo. This structure was used as the starting model for further refinements. The fit index and R factor for the starting model were poor (13.4% and 47%); subsequently, the fit converged to the second model shown in Table 1.
distances from the second in Table 1
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In protein crystallographic studies, which are typically carried out at ∼2 Å resolution, the presence of a heavy metal causes significant series-termination effects in the electron-density maps at about the same position as the position of the ligating atoms. The size of this ripple from the heavy metal is very significant compared with the scattering from the low-Z atom (Schindelin et al., 1997). The availability of higher-resolution crystallographic structures will help further clarify this aspect. In the absence of atomic resolution (1.2 Å or better), a three-dimensional combined with a high-resolution structure (∼2 Å) is a useful approach for obtaining highly precise stereochemical information. This is of particular importance when the effects of substrate binding or redox reactions are investigated and when subtle structural changes are expected to occur. An approach similar in spirit to the one described here was taken by Chen et al. (1993). They refined a model of the Cp1 FeMo-cofactor using single-crystal Mo and Fe although the major issues in their report were on positioning of the heavy atoms, whereas our emphasis is on positioning of the light atoms and the comparison between the cofactors in the MoFe and VFe proteins
3.2. V-nitrogenases
The VFe proteins from Azotobacter vinelandii (Av1v) (Hales et al., 1986) and A. chroococcum (Ac1v) (Eady et al., 1987) have been purified and characterized to show that they generally have very similar properties. The focus of much of the work on these systems has been on the types of redox centres that are present in the proteins and how the presence of V changes the catalytic properties compared with Mo-nitrogenase. (EPR), (CD) and Mössbauer studies of these proteins are consistent with them containing P clusters and a cofactor centre analogous to FeMoco but containing V. These spectroscopic data fit well with the genetic and sequence data discussed above, which predict that such centres are present in VFe proteins (see Eady, 1996). Further supporting evidence for the presence of cofactor binding sites is provided by the ability of isolated FeMoco to bind and activate apo-Av1v (Moore et al., 1994).
The detail of the environment of V in these cofactor centres, in the absence of a v was initially compared with the model compound [Me4N][VFe3S4Cl3(DMF)3]. This study, the first application of V K-edge X-ray absorption spectroscopy to a biological system, indicated that the V atom had a distorted octahedral symmetry and was in a similar environment to that in the model compound (Arber et al., 1987). The data for Av1v are very similar (George et al., 1988), and the region for both proteins can be simulated by a three-component fit with Fe, S and O as nearest neighbours to the V atom. This assignment and the distances of the atoms from the V atom are very similar to those for Mo in the FeMoco centre of MoFe proteins. In the case of MoFe proteins, the X-ray structure removed the ambiguity as to the nature of the light atoms; the Mo is coordinated to the carboxyl and hydroxyl groups of homocitrate and an N atom of the imidazole of His442. As discussed above, both homocitrate and the corresponding His residue are implicated in V-nitrogenase function, allowing a fairly safe assignment of the light atoms in the first coordination shell of the V atom.
of a VFe protein, has been provided from studies. The V of Ac1Conditions were subsequently established that allowed the putative FeVco centres to be extracted into NMF in a form capable of activating MoFe apo-protein produced by mutant organisms defective in FeMoco biosynthesis (Smith et al., 1988). The successful extraction of FeVco allowed the Fe of the isolated cofactor to be determined and compared with the data for Av1v (Harvey et al., 1990). In this case, the is dominated by Fe—S and Fe—Fe interactions at 2.32 and 2.64 Å, respectively. A longer Fe–Fe distance at 3.7 Å is also required in the simulation in all three cases. Fig. 4 shows the close similarity of both the data and the Fourier transforms of the two cofactors and emphasizes their structural similarity.
4. Conclusion
Z ligands, histidine and homocitrate. The three-dimensional of the data has shown that, even though these ligands are clearly seen in the their metrical data at 1.6 Å resolution suffer from an ambiguity that probably arises from the presence of the heavy metal, Mo. This example demonstrates the interplay between the X-ray absorption and diffraction techniques. For medium (< 1.5 Å) resolution structures, we recommend using metrical information derived from as soft restraints in crystallographic refinement.
has played a key role in the structural characterization of the nitrogenase cofactors. For MoFe nitrogenases, the first structural information on FeMoco was provided by in 1978 and demonstrated the presence of a cubane-type cluster. The overall organization of this cluster was confirmed by the in the early 1990s, with clear identification of the presence of the low-Acknowledgements
This work was supported by the BBSRC as part of the competitive strategic grant to JIC. We would like to thank Professor Barry Smith for his enthusiastic help and advice during this project.
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