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Figure 3
(a) A solution of 2.8 mM zinc in the presence of 7.1 mM D-PEN (ratio 1:2.5) in 50 mM Hepes buffer at pH 7.1 is mixed with 2.8 mM EDTA in the same buffer at 298 K. XAFS scans of the reaction are shown for 4 s (dashed line), 33, 106, 281 and 573 s after mixing. (b) The time course of the absorption ratio of the XAFS intensities at 9676 and 9682 eV (the latter is one of the isobestic points of Fig. 2[link]), reflecting the replacement of D-PEN by EDTA in the D-PEN complex of zinc.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 11| Part 2| February 2004| Pages 204-208
doi:10.1107/S0909049503028474
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