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Figure 2
Chromatogram of the scattering as the protein, cytochrome c, eluted from the column. The forward scatter was extrapolated from the Guinier lines, and provides a measure of the total mass in the beam, weighted by its molecular size. We see a single major peak emerging at approximately 56 min. The radius of gyration of the eluted material is constant at 1.3 nm throughout this peak, when a cut-off of 10 photons per pixel is used as a reliability criterion. Small amounts of aggregate or other high-molecular-mass impurities are seen in the 43–50 min range; as expected, larger Rg values were yielded from these data sets, although the scattered intensity is so low that these results are not deemed especially reliable (the symbols are left open for these points). The arrows in the lower part of the figure indicate the regions from which the individual Guinier plots of Fig. 4[link] were obtained.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 11| Part 4| June 2004| Pages 314-318
doi:10.1107/S0909049504014086
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