Figure 4
Guinier plots of individual images along the chromatogram shown in Fig. 2 (indicated by the arrows in that figure and ordered by the image numbers to the right of this figure) show good linearity, and are strongly parallel, indicating that one unique species is present. Note that the lowest-intensity data set spans intensities of <10 photons per pixel, and yet still yields a consistent and reliable Rg. This corresponds to concentrations <200 µg ml−1. |