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Figure 5
A chromatograph as plasminogen eluted from the column, showing not only scattering parameters Rg and I′(0) but also the UV absorption at 280 nm as a measure of concentration. A major peak at 36 min was observed in both A280 and I′(0), but substantial high-molecular-mass species were observed in the I′(0) trace in the region immediately preceding this, 20–36 min. Since I′(0) is proportional to both molecular mass and concentration, whereas A280 is proportional to only concentration, the differences in these two traces may be ascribed to differences in molecular mass. This is confirmed in the Rg data, which shows somewhat of a plateau at ∼3.5 nm in the major peak region, but much higher and more irregular values in this putative oligomer region. The filled symbols for the Rg trace correspond to the major peak, as defined by the region where the A280/I′(0) ratio remains constant, while the open symbol correspond to the putative aggregate region. The points are similarly delimited in Fig. 7[link].

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 11| Part 4| June 2004| Pages 314-318
doi:10.1107/S0909049504014086
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