Figure 7
The major plasminogen peak shows strongly non-ideal behaviour, with Rg varying as the concentration changes. This can be seen when either I′(0) or A280 are used as a proxy of concentration. The filled symbols are data points in the major peak region of Fig. 5, and were used to perform the linear extrapolation to [plasminogen] = 0. The open symbols are data points in the earlier aggregate region. The two different proxies for concentration yield a consistent Rg0 = 3.4 nm [for A280: 3.48 ± 0.07; for I′(0): 3.37 ± 0.06]. |