journal menu
![[Figure 2]](he5304fig2mag.jpg)
|
|
Figure 2
(a) Composite SAXS/WAXS pattern of the Pex5p(C)/mSCP2 complex. SAXS data (0.018 Å−1< s < 0.45 Å−1) and WAXS data (0.25 Å−1 < s < 0.90 Å−1) have been merged. The protein sample was at 13 mg ml−1 in 10 mM potassium phosphate, pH 7.4, and 1 mM DTT. Raw scattering data are shown as open circles, with error bars. Data fits for ab initio models restored by DAMMIN (Svergun, 1999  ) (red line, final χ value against the raw data = 0.6145) and GASBOR (version 18) (Svergun et al., 2001) (blue line, χ = 0.8571) are shown. The rigid-body model fit from MASSHA is shown as a green line, χ = 2.09. (b) Guinier plots for Pex5p(C)/mSCP2 (circles) and Pex5p(C)/preSCP2 (triangles). Only the Pex5p(C)/mSCP complex shows a linear s−2/lnI relationship, indicative of a monodispersed population of scattering particles (Koch et al., 2003). (c) Real-space fit to p(r) using GASBOR (version 20) (Petoukhov & Svergun, 2003). p(r) output from GNOM (Svergun, 1992) is shown in black and the model fit in red. dmax is 90 Å. The final χ value against the raw data is 0.7637. |
![[Figure 2]](he5304fig2.jpg)
 | JOURNAL OF SYNCHROTRON RADIATION |
ISSN: 1600-5775
