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![[Figure 4]](ml5204fig4mag.jpg)
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Figure 4
An X-ray diffraction study on skeletal muscle contraction. (a) A meridional region of the diffraction pattern from an isometrically contracting frog muscle. In the centre, a backstop and a scatter are observed. The two strong peaks on both sides are the third-order meridional reflections from the myosin filaments (at 1/145 Å−1). The exposure time was 0.53 ms. The image size was 640 × 72 pixels. The temperature was 278 K. (b) The intensity change of the myosin meridional reflection (14.5 nm) after a quick release of the muscle with an amplitude of about 1%, which completes in 1 ms. The data set was obtained in a single run. The fluctuation seen during the intensity recovery is probably due to changes in the muscle thickness: since the muscle was moved continuously to avoid radiation damage during the experiment, the part of the muscle that was irradiated changed continuously and the muscle thickness may vary with time (Yagi, 2003  ). (c) Timing diagram to show how a time resolution higher than the frame rate can be achieved by using a rotating shutter. As the shutter is synchronized with the frames, it is possible to pass the X-rays at the same timing in all frames. By changing the delay time and combining all data afterwards, it is possible to obtain continuous time-resolved data with an exposure time shorter than the frame length. |
![[Figure 4]](ml5204fig4.jpg)
 | JOURNAL OF SYNCHROTRON RADIATION |
ISSN: 1600-5775
