Figure 2
Purification profile of azurin I. SDS-PAGE (15% w/v polyacrylamide) analysis of various stages of protein purification is shown. Lane S, molecular mass standards; lane 1, whole cell lysate (50 µg protein); lane 2, crude cell extract (50 µg protein); lane 3, pool of DEAE flow-through (50 µg protein); lane 4, purified azurin I after CM-cellulose chromatography (5 µg protein). |