Figure 4
Purification of MTB proteins. 1: Protein from rv2547(His 6 tagged) after nickel affinity column purification; 2: protein from rv2547 after a second pass down a nickel affinity column; 3: protein from rv38365 after nickel affinity column purification; 4: protein molecular weight markers; 5: protein from rv3836 after nickel affinity and gel filtration; 6: protein from rv2547 after nickel affinity and gel filtration. |