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Figure 1
Schematic flowchart for the two alternate workflows for high-throughput X-ray absorption spectroscopy (HTXAS). The top workflow involves separation of genes by cloning, followed by HT expression and purification prior to organizing multiple samples for further analysis. The bottom workflow involves expression of the proteome prior to separation and organizing the multiple samples. Sample throughput can be accomplished either by spatial arraying or automated temporal sequencing. Synchrotron radiation analysis consists of mapping the metal distribution using X-ray fluorescence (XRF), using XANES for metal speciation and using EXAFS for metal-site structural analysis of the metalloproteome. Each arrow indicated on the flowchart represents a potential bottleneck for the overall high-throughput pipeline.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 12| Part 1| December 2005| Pages 19-22
doi:10.1107/S0909049504028791
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