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Figure 8
Microscope images of (a) insulin I and (b) insulin II after the crystals were warmed to 180 K at the end of the experiment. The white box indicates the beam dimensions, and the dark patch on the insulin I crystal shows the most damaged area, corresponding to the centre of rotation during the experiment. The area is no longer in the box as, on warming, the crystal moved into the cryobuffer. For the insulin II crystal, where the beam was vertically defocused, there is no visible damage.

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