Figure 2
Relative unit-cell volume (closed squares), temperature (open triangles) and integrated ice-ring intensities (open circles) as a function of experimental time for (a) an orthorhombic and (b) a trigonal trypsin crystal that were cryoprotected and transferred to mineral oil. The ice-ring intensities (2.3–2.19 Å) do not refer to either of the y axes, are on an arbitrary scale and were zero at 100 K prior to initiation of the warming–cooling procedure. |