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Figure 5
Microdiffraction pattern recorded from a quick-frozen myofibril of bumblebee flight muscle. (a) A series of diffraction patterns recorded by scanning the grid in the direction at 60° to the myofibril axis with 1 µm steps. Exposure time, 10 s each. (b) Intensity profile of the diffraction pattern measured along the equator. Black line, profile taken from one (middle) of the microdiffraction patterns in (a). Gray line, profile obtained from an end-on (powder) diffraction pattern recorded with a 50 µm pinhole (Iwamoto, Nishikawa et al., 2002BB7). The lack of the 1,1 reflection is evident in the profile taken from a single myofibril. (c) Intensity of the 2,0 reflection as a function of position on the grid, measured across the myofibrillar axis. Circles represent the measured data, which can be fitted to a sum of two Gaussian distributions (gray line). Solid lines are the distributions corrected for the beam width (Fig. 2[link]) by deconvolution. The values indicated beneath the horizontal lines are the FWHMs of the two distributions.

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