Figure 5
Microdiffraction pattern recorded from a quick-frozen myofibril of bumblebee flight muscle. (a) A series of diffraction patterns recorded by scanning the grid in the direction at 60° to the myofibril axis with 1 µm steps. Exposure time, 10 s each. (b) Intensity profile of the diffraction pattern measured along the equator. Black line, profile taken from one (middle) of the microdiffraction patterns in (a). Gray line, profile obtained from an end-on (powder) diffraction pattern recorded with a 50 µm pinhole (Iwamoto, Nishikawa et al., 2002). The lack of the 1,1 reflection is evident in the profile taken from a single myofibril. (c) Intensity of the 2,0 reflection as a function of position on the grid, measured across the myofibrillar axis. Circles represent the measured data, which can be fitted to a sum of two Gaussian distributions (gray line). Solid lines are the distributions corrected for the beam width (Fig. 2) by deconvolution. The values indicated beneath the horizontal lines are the FWHMs of the two distributions. |