Figure 6
Conventional diffraction pattern from a quick-frozen single skeletal muscle fiber in rigor, recorded at BL45XU. Background scattering has been subtracted according to the method described by Iwamoto et al. (2003). The right half of the pattern is shown with a higher gain. The black overlapping rings at the center are due to the correction for a circular aluminium mask to attenuate the central intense part of the diffraction pattern. The outer whitish ring is due to the background subtraction. The four quadrants of the diffraction pattern have been folded. Note that a number of actin-based layer-line reflections (first to seventh) are clearly visible (arrows), including the faint outer second actin layer line reflection (TM). Total exposure time, 500 s. |