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![[Figure 3]](kv5017fig3mag.jpg)
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Figure 3
SERCA phase behavior. Filled circles correspond to precipitated conditions and open circles correspond to non-precipitating conditions. (a) Phase behavior of SERCA as a function of PEG 1500 concentration with 0.1 M 1:2:2 malic acid, MES, TRIS linear buffer pH 6.5 (Newman, 2004  ), 0.1 M calcium acetate and 8% v/v glycerol. The colored squares are the points in the phase diagram where sitting-drop experiments were intended to equilibrate. The experiments represented by the red and yellow squares resulted in small rod-shaped crystals emanating from phase separation; those represented by the green square gave thin plates clusters. (b) Phase behavior of SERCA as a function of PEG 1500 concentration with 0.1 M 1:2:2 malic acid, MES, TRIS linear buffer pH 6.5, 0.1 M potassium phosphate and 8% v/v glycerol. Sitting-drop crystallization experiment equilibration points are represented by the colored squares: the experiment represented by the yellow square remained clear, microcrystals and thin plates grew from the experiments represented by the green and red squares, and the experiment represented by the turquoise square resulted in amorphous precipitation. |
![[Figure 3]](kv5017fig3.jpg)
 | JOURNAL OF SYNCHROTRON RADIATION |
ISSN: 1600-5775
