Figure 4
Effect of pH on phase behavior of UMP kinase. Filled circles correspond to precipitated conditions and open circles correspond to non-precipitating conditions. (a) Precipitation diagram for UMP kinase and PEG 8000 at pH 4 with 0.1 M 1:2:2 malic acid, MES, TRIS linear buffer and 50 mM magnesium formate. (b) Precipitation diagram for UMP kinase and PEG 8000 at pH 5 with 0.1 M 1:2:2 malic acid, MES, TRIS linear buffer and 50 mM magnesium formate. (c) Precipitation diagram for UMP kinase and PEG 8000 at pH 6 with 0.1 M 1:2:2 malic acid, MES, TRIS linear buffer and 50 mM magnesium formate. |