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![[Figure 5]](kv5017fig5mag.jpg)
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Figure 5
Effect of pH on crystal morphology of UMP kinase. The scale bar is 200 µm on all pictures. (a) Initial crystal hit, crystals grown from 0.8 mg ml−1 UMP K and 12.5% w/v PEG 8000 with 0.1 M MES pH 6 and 50 mM magnesium formate. (b) Small rectangular crystal grown at pH 6 in the presence of 1 mg ml−1 UMP K and 7.5% w/v PEG 8000 with 0.1 M 1:2:2 malic acid, MES, TRIS linear buffer and 50 mM magnesium formate. (c) Rectangular crystal and rod-shaped crystals grown at pH 5 in the presence of 0.7 mg ml−1 UMP K and 7.5% w/v PEG 8000 with 0.1 M 1:2:2 malic acid, MES, TRIS linear buffer and 50 mM magnesium formate. (d) Rod-shaped crystal grown at pH 4 in the presence of 1 mg ml−1 UMP K and 10% w/v PEG 8000 with 0.1 M 1:2:2 malic acid, MES, TRIS linear buffer and 50 mM magnesium formate. |
![[Figure 5]](kv5017fig5.jpg)
 | JOURNAL OF SYNCHROTRON RADIATION |
ISSN: 1600-5775
