Figure 4
(Top) In black, the spectrum of pure lysozyme from lyophilized powder dissolved in 0.1% TFA, and in grey, the spectrum of a marked lysozyme crystal. The three peaks were assigned as [m]+ for m/z = 14307.785, [m]2+ (double charge) for m/z = 7149.769 and [m]3+ (triple charge) for m/z = 4763.4763. There is no evidence of peak shifts. (Bottom) Low weights detail: in black, the spectrum of pure lysozyme, and in grey, the spectrum of a marked lysozyme crystal. The peak at 474 Da (CFSE) is evidently present only in the grey spectrum. |