Temperature dependence of the D½ of 31 mM SeMet in 0.6 M NaOH and 25% (v/v) glycerol. D½ values for samples of this solution were measured with the sample cooler set for different temperatures (error bars). Lower temperatures continued to increase the D½ of SeMet down to ∼92 K which was the lowest temperature obtainable with this equipment. The solid line is a smooth curve (to guide the eye). There is a clear transition in the slope of the temperature dependence of the D½ around 130 K which may correspond to the glass transition of pure water near this same temperature, allowing new factors to come into play above 130 K.