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Figure 1
Cryoradiolytic reduction of met myoglobin crystals kept at 100 K. (a) Time course of the UV-Vis absorption spectrum of met myoglobin crystals during X-ray irradiation at 10 keV with a flux of 1.1 × 1012 photons s−1 and a focused beam. The temporal resolution is 50 ms. (b) Myoglobin absorption spectra taken before and after certain time-points after starting the X-ray irradiation. A new species forms rapidly (dose 0.2 MGy, red) after initiation of X-ray irradiation. This species reaches saturation after 15 s (dose 2 MGy, green). When approaching the dose limit (30 MGy; Owen et al., 2006BB65) the whole spectrum decays slowly (blue). (c) Time course of the 566 nm peak.

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