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![[Figure 1]](xh5009fig1mag.jpg)
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Figure 1
Quaternary structure of Hm MalDH in its apo-form and structural changes caused by radiation damage. Hm MalDH is a homotetrameric molecule; A–D identify the four monomers. The two tight dimers (A–B) and (C–D) delineate a cavity, filled by a network of over 100 ordered water molecules. Solvent channels are indicated by dashed lines. The regions with salt-bridge clusters (numbered 1–3, as described by Richard et al., 2000  ) are highlighted. Secondary structures are shown as ribbons, and side-chains of damaged acidic residues as sticks. Eight chloride ions, rendered as spheres, are localized at monomer interfaces. The Fo 3-Fo 1 difference electron density map (negative contours in red and positive contours in green drawn at ±5.0σ levels) is overlaid on the reference model 1. The active site (red circle, Asp 168) and the cavity display the highest negative peaks which correspond to the loss in electron density associated to the side-chains of the most radiation-damage-sensitive residues (high damage, see Table 3). |
![[Figure 1]](xh5009fig1.jpg)
 | JOURNAL OF SYNCHROTRON RADIATION |
ISSN: 1600-5775
