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Figure 6
Synchrotron footprinting of the Ca2+-dependent conformational activation of gelsolin. (a) Subunit structure of gelsoline. Individual colors correspond to specific subdomains. (b) Peptides that are oxidized but the extent of oxidation does not change as a function of Ca2+ concentration. The peptide locations are color coded according to the subdomain where they are found as per (a). (c) Peptides that were sensitive to Ca2+ concentration. The peptide locations are color coded according to the subdomain where they are found as per (a). (d) Representative Ca2+ titration isotherm for the peptide p431–454 and p722–748 in domains S6 and S4, respectively. Each symbol is generated from a single experiment showing the extent of oxidation after exposure of gelsolin to X-rays for 80 ms at a specific concentration of Ca2+. Reproduced with permission from Kiselar et al. (2003bBB14).

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 14| Part 3| April 2007| Pages 233-243
doi:10.1107/S0909049507013118
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