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Figure 3
Molecular functional group peak area ratio: area under the protein amide I (1650 cm−1) bands divided by the area under the peaks between ∼1180 and 950 cm−1 at each pixel (pixel size 10 µm ×10 µm) representing protein-to-total-carbohydrate ratio in the wheat seed tissue. (a) Visible image. (b) Spectra corresponding to the pixel at the cross-hair in the visible image. (c) Chemical ratio image: protein to carbohydrate ratio. (d) Region and baseline of protein amide I and II and carbohydrate. (e) Three-dimensional image. (f) Ratio image profile set-up.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 14| Part 4| July 2007| Pages 382-390
https://doi.org/10.1107/S0909049507014264
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