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Figure 2
Three-dimensional reconstruction of the voltage-sensitive sodium channel from cryo-EM images. (a) Raw images of the sodium channel with different Euler angles. (b) Corresponding class averages (two-dimensional averages) for each raw image. An improved signal-to-noise ratio revealed characteristic views of the sodium channel. (c) Corresponding projection images from the three-dimensional structure and (d) surface views of the three-dimensional reconstruction. (e) EM of negatively stained sodium channel–antibody complexes. The cytoplasmic region was assigned by the binding of the antibody against the N-terminal sequence of the channel protein. The densities of the channel proteins and antibodies are indicated in the figures by ch and Ab, respectively. The scale bars represent 50 Å.

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