Figure 3
A schematic representation of the process used for perdeuterating proteins at the BDL at Los Alamos National Laboratory (LANL). A bacterial culture medium, which we designate Altone, is made from the hydrolyzate of algae (e.g. Scenedesmus obliquus) grown in D2O and used for protein expression in E. coli. Steps represented by framed boxes can be performed robotically at LANL. Steps represented by shaded boxes can be performed either at LANL or the user's home laboratory. |