Figure 5
Integrated scattering intensity profiles from ice from the specimens frozen in the presence or absence of various cryoprotectants, taken from the WAXS recording as shown in Fig. 3. (a) Without cryoprotectant; (b) 20% sucrose; (c) 20% glycerol; (d) 5% MPD; (e) 10% MPD; (f) 20% MPD; (g) 20% DMSO. The baselines of the profiles from different depths (indicated on the left in µm) are offset for clarity. The small spikes in (f) originate from the sporadic reflections from ice crystals probably generated in the process of specimen trimming after freezing. |