Figure 3
BBB permeability. Two-photon fluorescence microscopy images of RhodamineB-dextran 70 kDa (column I), FITC-dextran 4 kDa (column II) and merge of the two images (column III). Images were acquired at 150 µm below the dura in a healthy mouse (A–C); in a mechanically BBB induced disruption model (D–F); 48 h post irradiation (G–I); one week post irradiation (J–L) and one month post irradiation (M–O). Note the black holes (arrows, E–F) which are cells not labelled by the extravasated dye. |