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Figure 2
Example of high- and low-contrast IR images from soft tissues. The top series represents visible (left), full spectral intensity (centre; averaged intensity absorption of the 4000–500 cm−1 spectral interval) and lipid/amide ratio (right; intensity absorption of the 1700–1500 cm−1 spectral interval) images of skeletal muscle fibres from fresh tissue (20 µm section). The red square in the visible image is the area analyzed by FTIR microspectroscopy. The characteristic spatial arrangement of fibres and connective tissue in the muscle allows high-contrast IR images with spectra highlighting the differences in molecular composition to be obtained. The bottom series represents visible (left), full spectral intensity (centre; averaged intensity absorption of the 4000–500 cm−1 spectral interval) and glucose IR absorption (right; distribution of the 1033 cm−1 absorption band intensity) images of a rat brain tumor (tissue section of 20 µm).

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 17| Part 1| January 2010| Pages 1-11
https://doi.org/10.1107/S0909049509046056
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