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Figure 4
Graphical user interface used for irradiation field positioning in the rat head. The horizontal white line represents the beam and the rotation axis positions (defined by line 4). The vertical white line corresponds to the central microbeam y position (defined by line 5). The width and height of the irradiation field (grey on the X-ray image) are defined by line 2. For line 1, the user can also indicate an offset distance (4 mm from the skull surface in this case, white dashed arrow) between the centre of the irradiation field and a given anatomical reference (bregma, black arrow). Two motor motions in the y and z directions are required to move the target into the beam. These are automatically calculated when positioning the radiation target and are given by line 3 (y motion) and line 6 (z motion).

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