Figure 10
Detection limit of the BL04B (SNM) VUVCD end-station. All spectra were recorded with slit width 300 µm, time constant 10 s, single scan and without smoothing. Upper panel: spectra of aqueous myoglobin (0.57 and 1.15 mg ml−1) in a CaF2 cell (path length 7 µm, H2O baseline subtracted), representing samples with a `weak signal'. Lower table: results of online analysis (DichroWeb) of protein secondary structure calculated from spectra of myoglobin at weak signals (0.57 and 1.15 mg ml−1) and signals from regular concentrations (2.25 mg ml−1, 4.5 mg ml−1 and 16.5 mg ml−1). |