Figure 8
Spectra of myoglobin dissolved in H2O, D2O and HFIP at 298 K (solvent baseline subtracted, triplicate scans averaged). The spectra were recorded with a CaF2 cell (path length 7 µm), wavelength increment 0.6 nm and time constants 1 s for a sample in D2O and H2O or 10 s for its counterpart in HFIP. The inset shows the voltage of the PMT recorded for each sample over the measured range of wavelength. |