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Figure 1
DNA double-strand break repair in V79 cells after MRT pattern emulation. Fluorescent staining with the γH2AX antibody (a marker of DNA damage) shows synchrotron MRT emulation in cell culture using a slit system of varying beam widths and interbeam spacings. The in-air entrance dose was 10 Gy in all cases and the cells (V79 Chinese hamster lung cells) were fixed 1 h (ae) and 12 h (fj) post-irradiation. (a, f) 25 µm beam width with centre-to-centre spacings of between 50 and 300 µm, (bg) 50 µm beam width with spacings of between 75 and 300 µm, (c, h) 100 µm beam width with a 200 µm centre-to-centre spacing, and (d, i) 150 µm beam width and a 200 µm spacing. (e, j) Zoomed images from 100 µm beam width with a 200 µm centre-to-centre spacing. Bars indicate 100 µm.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
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