Figure 3
The DNA damage response of irradiated V79 cells. V79 cells were exposed to 10 Gy (a), 5 Gy (b) and 2 Gy (c), using MRT patterned 5 keV X-rays and stained for γH2AX 1 h after irradiation. The microbeam widths were 100 µm with a centre-to-centre spacing of 200 µm. Individual nucleus intensity was quantified by determining the relative fluorescence using a separate targeted single-cell irradiation (d). Intensities were quantified using GeneTools (Syngene, Cambridge, UK). Error bars are standard error of the mean of four cell nuclei for each dose. Bars indicate 100 µm. |