Figure 4
Partial cell irradiation with a 25 µm-wide beam of (a–h) N1-RGB cells and (i–p) V79 cells reveals clear demarcation of radiation-induced DNA double-strand breaks as determined by γH2AX foci. The in-air entrance dose was 10 Gy. Arrowheads point to γH2AX foci outside the apparent zone of irradiation. The brightness and contrast settings for some images (c and l) have been adjusted to compensate for staining variation. Some cell images are at a slightly higher zoom (l). |