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![[Figure 1]](wa5026fig1mag.jpg)
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Figure 1
Schematic view of the key hardware components of the sample changer (a). It consists of a motorized XYZ translation arm (A), a syringe needle (B), a temperature-controlled sample rack in the 96-well microplate format (C), a temperature-controlled capillary holder assembly (D) and a quartz capillary. The capillary holder assembly (D), made of anodized aluminium, interfaces the capillary to compressed air via a valve for drying, as well as two syringes, for capillary cleaning and sample aliquot handling. The gentle compression of the syringe needle holder onto the capillary holder assembly makes an air-tight O-ring seal, enabling precise positional control of the sample aliquot within the quartz capillary. The inset (b) depicts the capillary holder assembly in detail. It consists of three parts: an air valve (D1) that switches the compressed air flow for drying the capillary, an interface part (D2) with internal channels (as outlined in the drawing) connecting the capillary to the cleaning syringe, the air valve and the syringe needle, and a capillary holder (D3) into which the quartz capillary is glued. The O-rings sealing the connections between (D2) and (D3) as well as between (D2) and the syringe needle holder are also depicted. Samples are placed on the sample rack in two different patterns (c) for dilution series (columns 1 and 2) or for titration series (columns 4 and 5): the white wells represent buffer solutions, the coloured wells protein samples and the blue wells empty. |
![[Figure 1]](wa5026fig1.jpg)
 | JOURNAL OF SYNCHROTRON RADIATION |
ISSN: 1600-5775
